Trainer Bob Baffert has been suspended for two years by Churchill Downs after an additional drug test of Medina Spirit confirmed the presence of the steroid betamethasone in the Kentucky Derby winner's system.
Disqualification of Medina Spirit could be next. The Kentucky Horse Racing Commission is still completing its investigation before making that ruling.
But Baffert will not be able to enter any horses in the Kentucky Derby or other races at the storied Louisville track through the spring of 2023.
Churchill Downs says it reserves the right to extend Baffert's suspension if he has any other violations in other states. Baffert has had five in the past 13 months.
Maryland racing officials allowed Medina Spirit and Baffert-trained Concert Tour to run in the Preakness on May 15 only after undergoing three rounds of pre-race testing, while New York banned Baffert indefinitely and prevented him from entering any horses in the Belmont Stakes.
"Reckless practices and substance violations that jeopardise the safety of our equine and human athletes or compromise the integrity of our sport are not acceptable, and as a company we must take measures to demonstrate that they will not be tolerated," Churchill Downs said.
Earlier lawyers for Baffert and Medina Spirit owner Amr Zedan confirmed the split-sample test came back positive for betamethasone.
Baffert's lawyer, Craig Robertson, said additional testing is being conducted to try to trace the source of the drug to an ointment to treat a skin infection and not an injection.
He expects tests to show the ointment is responsible and not injections into one of the horse's joints.